cold water fish skin gelatin (cwfg Search Results


anti pthr  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti pthr
Anti Pthr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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rabbit anti cfos 9f6 primary monoclonal antibody  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc rabbit anti cfos 9f6 primary monoclonal antibody
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Rabbit Anti Cfos 9f6 Primary Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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cwfs  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research cwfs
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Cwfs, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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pbg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology pbg
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Pbg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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cold water fish skin gelatin in tbst  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology cold water fish skin gelatin in tbst
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Cold Water Fish Skin Gelatin In Tbst, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cwfg  (Nanoprobes Inc)
86
Nanoprobes Inc cwfg
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Cwfg, supplied by Nanoprobes Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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chemie wirtschaftsförderungsgesellschaft mbh  (Chemie GmbH)
90
Chemie GmbH chemie wirtschaftsförderungsgesellschaft mbh
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Chemie Wirtschaftsförderungsgesellschaft Mbh, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cwf 1200 furnace  (Carbolite Gero)
96
Carbolite Gero cwf 1200 furnace
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Cwf 1200 Furnace, supplied by Carbolite Gero, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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tlr2 agonist- cell wall fraction of h37rv (cwf)  (BEI Resources)
90
BEI Resources tlr2 agonist- cell wall fraction of h37rv (cwf)
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Tlr2 Agonist Cell Wall Fraction Of H37rv (Cwf), supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cool white fluorescent cwf tubes  (Philips Healthcare)
86
Philips Healthcare cool white fluorescent cwf tubes
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Cool White Fluorescent Cwf Tubes, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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unspecified drug target nootropic agent  (JW Pharmaceutical)
90
JW Pharmaceutical unspecified drug target nootropic agent
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
Unspecified Drug Target Nootropic Agent, supplied by JW Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flovan® cwf  (Huntsman International LLC)
90
Huntsman International LLC flovan® cwf
Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = <t>cFos).</t> (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.
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Image Search Results


Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = cFos). (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.

Journal: Frontiers in Molecular Neuroscience

Article Title: A localized tracing technique to explore intra-amygdala functional and structural correlates of individual variability in behavioral response

doi: 10.3389/fnmol.2025.1347539

Figure Lengend Snippet: Structural and functional connectivity patterns within the amygdala complex do not mediate individual variability in stress response. (A) Viral tracing and fluorescent labeling scheme show colors associated with each additional fluorescent marker (red = TVA-mCherry-G, green = oG-rabies-GFP, magenta = cFos). (B) Example triple-channel image of the amygdala region (red = mCherry, green = GFP, magenta = cFos). Top inset (pink box and outline) shows examples of a structural projection neuron (open pink arrow, GFP + cFos − ) and a functional projection neuron (closed pink arrow, GFP + cFos + ). Bottom inset (yellow box and outline) shows examples of a starter neuron (closed yellow arrow, GFP + mCherry + ) and a projection neuron (open yellow arrow GFP + mCherry − ). (C) Map of all identified starter cells (GFP + mCherry + ). (D) Correlations between starter cell count in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.835, p = 0.00263; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). Starter cell counts did not predict behavioral phenotype in any identified brain region. (E) Map of all identified structural (GFP + cFos − ) and functional (stress-activated) projection (GFP + cFos + ) neurons. (F) Correlations between structural projection neuron (GFP + cFos − ) count (striped) and functional projection neuron (GFP + cFos + ) count (solid) in each region and SI ratio with FDR-corrected and non-FDR-corrected significance threshold indicated (solid horizontal line, Pearson R = 0.749, p = 0.0125; dashed horizontal line, Pearson R = 0.632, p = 0.05; respectively). No significant correlations identified. (G) Correlations between overall number of activated cells (cFos + ) per brain region and SI ratios.

Article Snippet: Sections were serially stained with rabbit anti-cFos (9F6) primary monoclonal antibody (Cell Signaling, catalog number: 2250S, lot number: 12, 1:2,000 in 0.3% PBST + 5% Normal Goat Serum + 1% BSA + 0.2% Cold Water Fish Skin Gelatin block solution) and Alexa Fluor-conjugated AffiniPure goat anti-rabbit secondary antibody (Alexa Fluor-647, Sigma, code number: 111-605-033, lot number: 157045, 1:500 in 0.3% PBS-T).

Techniques: Functional Assay, Labeling, Marker, Cell Counting